Journal: Journal of translational medicine

Article Title: Notch3 enhances the synergistic effect of all-trans retinoic acid and calcipotriol in pancreatic stellate cell activation.

doi: 10.1186/s12967-025-06666-1

Figure Lengend Snippet: Fig. 9 ATRA and calcipotriol are independent of RARβ and VDR to regulate Notch3. Western blot analysis of the expression of Notch3 and the nuclear receptors RARβ and VDR after LE135 binding to ATRA or calcipotriol. A LE135 binding to ATRA for incubation of quiescent or activated PSCs (n = 3, *p < 0.05, **p < 0.01, ***p < 0.001). B Results of LE135 binding to calcipotriol for the incubation of quiescent or activated PSCs (n = 3, *p < 0.05, **p < 0.01, ***p < 0.001)

Article Snippet: The LE135 RAR antagonist was purchased from MedChemExpress (MCE), dissolved in DMSO at 50 mg/ml, and stored at − 80 °C.

Techniques: Western Blot, Expressing, Binding Assay, Incubation

Journal: Biomedicines

Article Title: Histone H3 Acetylation Is Involved in Retinoid Acid-Induced Neural Differentiation through Increasing Mitochondrial Function

doi: 10.3390/biomedicines11123251

Figure Lengend Snippet: RA promotes histone acetylation through RA signaling during neural differentiation of SH-SY5Y cells. ( a – c ) RT-qPCR analysis of relative mRNA expression of RARα , RARβ , and RARγ in SH-SY5Y cells after RA treatment for 3 and 7 days. n = 3; ** p < 0.01; one-way ANOVA. ( d ) Co-immunoprecipitation was performed in SH-SY5Y cells cultured in maintenance medium or stimulated with RA for 3 and 7 days. Immunoprecipitation was conducted using anti-RARα followed by Western blot analysis of PCAF, CBP, and P300. ( e – g ) Western blot analysis of H3K14ac and H3ac in SH-SY5Y cells treated with DMSO or RA or pretreated with BMS195614/LE135 for 24 h followed by RA for 3 days. n = 3; * p < 0.05; one-way ANOVA.

Article Snippet: To identify the roles of key enzymes and receptors during neural differentiation, the HAT activity inhibitor C646 (Sigma, St. Louis, MO, USA), RARα antagonist BMS195614, and RARβ antagonist LE135 (Glpbio, Montclair, CA, USA) were supplemented during the RA treatment.

Techniques: Quantitative RT-PCR, Expressing, Immunoprecipitation, Cell Culture, Western Blot

Journal: Biomedicines

Article Title: Histone H3 Acetylation Is Involved in Retinoid Acid-Induced Neural Differentiation through Increasing Mitochondrial Function

doi: 10.3390/biomedicines11123251

Figure Lengend Snippet: Inhibition of RA signaling downregulates mitochondrial function and blocks neural differentiation. SH-SY5Y cells were treated with DMSO or RA or pretreated with BMS195614/LE135 for 24 h followed by RA for 3 days. ( a – d ) RT-qPCR analysis of relative mRNA expression of mitochondrial function-related genes ( NDUFS3 , ANT1 , ANT2 , ANT3 ). n = 3; * p < 0.05, ** p < 0.01; one-way ANOVA. ( e ) Changes in morphology and expression of neuronal differentiation markers Tuj1 (green) and MAP2 (red). Scale bar, 50 µm. Images in the black box show cells at a higher magnification. Red or white arrows show representative changes in morphology. ( f – h ) Differentiation rates and mean fluorescence intensity of Tuj1 and MAP2. n = 3; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; one-way ANOVA. ( i , j ) RT-qPCR analysis of relative mRNA expression of the neural differentiation markers Tuj1 and MAP2 . n = 3; * p < 0.05, ** p < 0.01; one-way ANOVA.

Article Snippet: To identify the roles of key enzymes and receptors during neural differentiation, the HAT activity inhibitor C646 (Sigma, St. Louis, MO, USA), RARα antagonist BMS195614, and RARβ antagonist LE135 (Glpbio, Montclair, CA, USA) were supplemented during the RA treatment.

Techniques: Inhibition, Quantitative RT-PCR, Expressing, Fluorescence